Sequencing & PCR
Sequencing
Genetic analyzer (sequencer)
NANOPHORE® 05 (Syntol, Moscow)
NANOPHORE® 05 is four colors laser-induced fluorescent capillary electrophoresis system. Using NANOPHORE® 05 it is possible automatically determine DNA sequence as well as DNA fragments lengths.
Detector: Fluorescence detector at wavelengths 520, 550, 580 and 607 nm
Laser: Solid-phase, l = 488 nm, power Р = 1-10 mW
Separation efficiency: Single base separation efficiency for single stranded DNA fragments up to 600 nucleotides length with more than 98% accuracy
Time of analysis, hours: 2-2.5
High-voltage supplier: Voltage: 15 kV, currency: 1–150 μА , instability: 0.03 % max
Sample input: Electrokinetic
Sample plate: 96-samples plate, 12 places for additional reagents with volume from 0.5 to 3 mL
Capillary: 8 standard coated capillary, internal diameter 30–80 μm, length 360-500 mm
Thermostat: Peltier based thermostat, capillary temperature set from 15 to 65 ( ± 0.2)° С
Working regime: Automatic reload of gel under pressure up to 10 atm. Automatic probe separation using user made program with real time monitoring of main parameters of eхperiment