Micromanipulations & Vectors' Delivery
Transfection Systems
Thermo Fisher Scientific Neon Transfection System
The Neon™ Transfection System is a novel, benchtop electroporation device that employs an electroporation technology by using the pipette tip as an electroporation chamber to efficiently transfect mammalian cells including primary and immortalized hematopoietic cells, stem cells, and primary cells.
The Neon™ Transfection System efficiently delivers nucleic acids, proteins, and siRNA into all mammalian cell types including primary and stem cells with a high cell survival rate. The transfection is performed using as few as 1 × 104 or as many as 5 × 106 cells per reaction using a sample volume of 10 µL or 100 µL in a variety of cell culture formats (60 mm, 6-well, 48-well, and 24-well).
Voltage range: 500–2,500 V
Input Pulse Width range: 1–100 ms
Input Pulse Number range: 1–10
The Neon™ Transfection System offers open and transparent protocols that are optimized for ease of use and simplicity. Optimized protocols for many commonly used cell types are also available at https://www.thermofisher.com/us/en/home/life-science/cell-culture/ transfection/neon-transfection-system/neon-transfection-system-cell-line-data.html to maximize transfection efficiencies for your cell types.
Bio-Rad Micro Pulser
The MicroPulser Electroporator is a simple yet versatile instrument that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms, e.g. Dictyostelium discoideum. Transformation efficiencies much higher than those obtained with chemical methods can be achieved.
Outputs
Waveform - decaying or truncated decaying exponential-decay with resistor capacitor time constant
Voltage and current: 3,000 V peak into >600 W load; limited at 100 A peak maximum.
Output voltage and pulse duration adjustment - Voltage adjustable in 200–3,000 V range with 10 V precision; 5 ms default or 1–4 ms with 0.1 ms precision.
Input voltage - 100–120 V or 220–240 V
Number of pulses - 1 or 2
Preset protocols - 5 bacterial, 4 fungal, 1 D. discoideum
Operating environment - 3.5–35°C
Micromanipulators
Eppendorf micromanipulator TransferMan NK
Integrated coarse and fine manipulator with proportional control via central joystick
Work range: 20 mm per axis
Programmable Z axis limit as defined injection level in order to avoid capillary breakage
Automated Home function for rapid capillary exchange
Adjustable injection speed
Adjustable injection angle for complex injection processes
Comfortable fine adjustment of work speed with positioning wheel
Storage of 3 independent work positions
Menue controlled programming via display
Save and download of user profiles
External control for automatic processes
Microinjectors
FemtoJet 4i
The Eppendorf electronic microinjector, the FemtoJet 4i, is a tool for reproducibly injecting small volumes into adherent cells and suspension cells. In combination with the TransferMan micromanipulator, it forms a system to facilitate and speed up injections workflow.
FemtoJet 4i can be used for genome editing of early embryos (Drosophila, zebrafish, mouse) and eukaryotic cells (including induced pluripotent stem cell (iPSC) and hematopoietic stem cells).
Programmable injection guarantees high reproducibility.
Inject and Clean function can be triggered on the device using the included foot control or optional hand control.
Independent pressure supply with built-in compressor.
Especially well suited for injection volumes from femtoliters to 100 pL.
Programmable parameters Injection time, injection pressure, compensation pressure
Injection time 0.01 s – 99.99 s; can be set in increments of 0.01 s.
Injection pressure 5–6,000 hPa (0.07-87 psi).
Compensation pressure 5–6,000 hPa (0.07-87 psi).
Clean function Maximum rinsing pressure of 6,000 hPa (87 psi).
Continuous flow function 5–6,000 hPa (87 psi); can be set in increments of 1 hPa (0.01 psi).
CellTram 4r Air
Manual pneumatic microinjector
The CellTram 4r Air is a pneumatic injector for oil-free micromanipulation and gentle holding of cells in suspension. It is highly suitable for uptake and injection of cells (e.g., of sperm or ES cells). For this application, its performance is comparable to that of oil-filled injectors. It can be used with all common micromanipulation systems yet they develop their full potential with Eppendorf manipulators.
Max. pressure 3,000 hPa
Min. adjustable volume < 100 nL
Pressure generation Cylinder/piston system, air-filled
Volume change per revolution 60 µL/600 µL (fine/ coarse rotary knob)
CellTram 4r Oil
Manual hydraulic microinjector
The CellTram 4r Oil is a hydraulic, oil-filled microinjector that provides more direct sample control and can generate higher pressures than an air system. It is optimized for leakage-free work and easy oil filling.
Applications
ICSI of mouse and large mammals Holding of sensitive oocytes, embryos or small organisms; Transfer of cells or organelles; Enucleation/nuclear transfer; (Xeno)transplantations; Removal of cells; Manual microinjection; Single cell picking.
Two rotary knobs of different transmission ratio (1:1 and 1:10) for the highest standards in terms of resolution, sensitivity and response behavior
Max. pressure 20,000 hPa
Min. adjustable volume < 1.5 nL
Pressure generation Cylinder/piston system, oil-filled
Volume change per revolution 1 µL/10 µL (fine/ coarse rotary knobes.